Intra- and inter-laboratory validation of an innovative huFcεRIα-RBL-2H3 degranulation assay for in vitro allergenicity assessment of whey hydrolysates
详细信息 查看全文
- 作者：Karen Knippinga ; b ; karen.knipping@danone.com" class="auth_mail" title="E-mail the corresponding author ; Manon van Roestc ; Laura Kruijssenc ; Mieke Smitsd ; Marc Teunisd ; Linda Coxe ; 1 ; Niels de Jonge ; 1 ; Peter J. Simonse ; 1 ; Louis Boone ; 1 ; Reiko Teshimaf ; Marjan Grosg ; Diane Keglera ; Johan Garssena ; b ; Lé ; on M.J. Knippelsa ; b ; Raymond Pietersc ; d
- 关键词：AAF ; amino acid-based formula ; ALA ; α-lactalbumin ; BLG ; β-lactoglobulin ; CM ; cow's milk ; CMA ; cow's milk allergy ; eHF ; extensively hydrolyzed formula ; FcεRI ; high affinity IgE receptor ; HA ; hypoallergenic ; IgE ; immunoglobulin E ; RBL ; rat basophilic leukemia ; pHF ; partially hydrolyzed formula
- 刊名：Toxicology in Vitro
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：33
- 期：Complete
- 页码：29-34
- 全文大小：822 K
文摘
- •
Mast cell degranulation assay suitable to distinguish allergenicity of hydrolysates.
- •
Intra- and inter-laboratory validation supports suitability of degranulation assay.
- •
Mast cell degranulation assay may contribute to replacement animal testing.
- •
Humanized mouse antibodies allow wider use to mast cell degranulation assay.
- •
Note: same lot no. for human IgE and anti-human IgE are used in the ring trial.