Understanding of real alternative redox partner of Streptomyces peucetius DoxA: Prediction and validation using in silico and in聽vitro analyses
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- 作者：Hemraj Rimal ; Seung-Won Lee ; Joo-Ho Lee ; Tae-Jin Oh ; tjoh3782@sunmoon.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cytochrome P450 ; Electron-transport system ; Homology modeling ; In silico analysis ; Streptomyces peucetius ATCC27952
- 刊名：Archives of Biochemistry and Biophysics
- 出版年：2015
- 出版时间：1 November 2015
- 年：2015
- 卷：585
- 期：Complete
- 页码：64-74
- 全文大小：2292 K
文摘
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Endogenous redox partner system of DoxA for the production of doxorubicin is purposed.
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In silico study show FDR2 and FDX1 as a best electron transfer partners.
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The optimum temperature and pH are 7.6 and 30 °C respectively for the catalytic activity of enzyme.