Molecular and functional characteristics of erbB2 in normal and cancer breast cells

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• 作者：Tommasi ; Stefania ; Fedele ; Vita ; Lacalamita ; Rosanna ; Crapolicchio ; Antonella ; Perlino ; Elda ; et. al.
• 关键词：Tyrosine kinase receptor ; Truncated peptides ; Single nucleotide polymorphism ; Mammary carcinoma ; Expression pattern ; Transmembrane domain ; SNP ; single nucleotide polymorphism
• 刊名：Cancer Letters
• 出版年：2004
• 出版时间：June 25, 2004
• 年：2004
• 卷：209
• 期：2
• 页码：215-222
• 全文大小：216 K

文摘

The expression pattern of erbB2 and its transmembrane polymorphisms (Ile⁶⁵⁴Val and Ile⁶⁵⁵Val) were investigated in a panel of human normal and neoplastic breast cell lines to evaluate whether the expression pattern was affected by changes in the gene structure. At least two peptides of lower molecular mass forms (95 and 68 kDa) than the holoreceptor (185 kDa), comprehensive of the tyrosine kinase domain, were detected in all cells. Both peptides were also phosphorylated, suggesting a functional role in signal transduction. The presence of the polymorphisms found in two cell lines was unrelated to the expression of the lower molecular mass proteins.