Forensic interlaboratory evaluation of the ForFLUID kit for vaginal fluids identification

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• 作者：Saverio Giampaoli ; Federica Aless ; rini ; Andrea Berti ; Luigi Ripani ; Ajin Choi ; Roselien Crab ; Elisabetta De Vittori ; Balazs Egyed ; Cordula Haas ; Hwan Young Lee ; Marie Korabecn谩 ; Fabrice Noel ; Daniele Podini ; Adriano Tagliabracci ; Alessio Valentini ; Vincenzo Romano Spica
• 关键词：Vaginal fluids ; mfDNA ; Bacteria ; Interlaboratory evaluation ; Forensic ; Multiplex PCR
• 刊名：Journal of Forensic and Legal Medicine
• 出版年：January, 2014
• 年：2014
• 卷：21
• 期：Complete
• 页码：60-63
• 全文大小：209 K

文摘

Identification of vaginal fluids is an important step in the process of sexual assaults confirmation. Advances in both microbiology and molecular biology defined technical approaches allowing the discrimination of body fluids. These protocols are based on the identification of specific bacterial communities by microfloraDNA (mfDNA) amplification. A multiplex real time-PCR assay (ForFLUID kit) has been developed for identifying biological fluids and for discrimination among vaginal, oral and fecal samples. In order to test its efficacy and reliability of the assay in the identification of vaginal fluids, an interlaboratory evaluation has been performed on homogeneous vaginal swabs. All the involved laboratories were able to correctly recognize all the vaginal swabs, and no false positives were identified when the assay was applied on non-vaginal samples. The assay represents an useful molecular tool that can be easily adopted by forensic geneticists involved in vaginal fluid identification.