Performance evaluation of the Artus hepatitis C virus QS-RGQ assay

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• 作者：Pierpaolo Paba ; Lavinia Fabeni ; Carlo Federico Perno ; Marco Ciotti ; ^{marco.ciotti@ptvonline.it}
• 关键词：HCV real-time PCR ; HCV viral load
• 刊名：Journal of Virological Methods
• 出版年：2012
• 出版时间：January 2012
• 年：2012
• 卷：179
• 期：1
• 页码：77-80
• 全文大小：240 K

文摘

Accurate determination of hepatitis C virus RNA level is essential for evaluating the response to antiviral therapy, to determine the duration of treatment, and to predict treatment outcome. Currently, two real-time based polymerase chain reaction assays are used widely to monitor the hepatitis C RNA level: the Abbott RealTime HCV assay and the Cobas Taqman HCV assay. Recently, a third assay has become commercially available: the Artus HCV QS-RGQ assay, which uses the QIAsymphony SP/AS platform for sample preparation and PCR-setup, and the Rotor-Gene Q for amplification and detection. In this study, the performance of the Artus HCV QS-RGQ assay was tested on 105 plasma samples and compared to that of the Cobas Taqman HCV assay. Linear regression analysis showed a good agreement between the two assays. A slightly better sensitivity was observed with the Cobas Taqman assay, while higher hepatitis C viral RNA levels were measured by the Artus HCV QS-RGQ assay in samples positive for hepatitis C genotypes 4. Taken together, the data suggest that the Artus HCV QS-RGQ assay is useful in a diagnostic setting. The combination with the versatile QIAsymphony SP/AS system may represent a major advantage for clinical virological laboratories aiming at optimizing their workflow.