Ferritin, a protein containing iron nanoparticles, induces reactive oxygen species formation and inhibits glutamate uptake in rat brain synaptosomes

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• 作者：Alex ; ra V. Alekseenko ; Tatyana V. Waseem ; Sergei V. Fedorovich
• 关键词：Synaptosomes ; Ferritin ; Nanoparticle ; Free radicals ; Glutamate uptake ; Glutamate release
• 刊名：Brain Research
• 出版年：2008
• 出版时间：19 November 2008
• 年：2008
• 卷：1241
• 期：Complete
• 页码：193-200
• 全文大小：508 K

文摘

Nanoparticles are currently used in medicine as agents for targeted drug delivery and imaging. However it has been demonstrated that nanoparticles induce neurodegeneration in vivo and kill neurons in vitro. The cellular and molecular bases of this phenomenon are still unclear. We have used the protein ferritin as a nanoparticle model. Ferritin contains iron particles (Fe³⁺) with size 7 nm and a protein shell. We investigated how ferritin influences uptake and release of [¹⁴C]glutamate and free radical formation as monitored by fluorescent dye DCFDA in rat brain synaptosomes. We found that even a high concentration of ferritin (800 μg/ml) did not induce spontaneous [¹⁴C]glutamate release. In contrast the same concentration of this protein inhibited [¹⁴C]glutamate uptake two fold. Furthermore ferritin induced intrasynaptosomal ROS (reactive oxygen species) formation in a dose-dependent manner. This process was insensitive to 30 μM DPI, an inhibitor of NADPH oxidase and to 10 μM CCCP, a mitochondrial uncoupler. These results indicate that iron-based nanoparticles can cause ROS and decreased glutamate uptake, potentially leading to neurodegeneration.