Peritoneal fluid: a potential mechanism of systemic neutrophil priming in experimental intra-abdominal sepsis

详细信息    查看全文

• 作者：Shinil K. Shah ; D.O.^a ; ^c ; Fernando Jimenez ; M.S.^a ; Peter A. Walker ; M.D.^a ; Hasen Xue ; M.D.^a ; Teri D. Feeley ; B.S.^b ; Karen S. Uray ; Ph.D.^a ; ^c ; Kenneth C. Norbury ; Ph.D.^b ; Randolph H. Stewart ; D.V.M. ; Ph.D.^c ; Glen A. Laine ; Ph.D.^c ; Charles S. Cox Jr ; M.D.^a ; ^c ; ^{charles.s.cox@uth.tmc.edu}
• 关键词：Trauma ; Resuscitation ; Abdomen ; White blood cells ; Neutrophil activation ; Intra-abdominal sepsis
• 刊名：The American Journal of Surgery
• 出版年：2012
• 出版时间：February 2012
• 年：2012
• 卷：203
• 期：2
• 页码：211-216
• 全文大小：458 K

文摘

Background

Recent studies suggest that peritoneal fluid (PF) may be an important mediator of inflammation. The aim of this study was to test the hypothesis that PF may drive systemic inflammation in intra-abdominal sepsis by representing a priming agent for neutrophils.

Methods

PF was collected 12 hours after the initiation of intra-abdominal sepsis in swine. Naive human neutrophils were primed with PF before treatment with N-formyl-Met-Leu-Phe or phorbol 12-myristate 13-acetate to elucidate receptor-dependent and receptor-independent mechanisms of neutrophil activation. Flow cytometry was used to quantify neutrophil surface adhesion marker expression of integrins and selectins and superoxide anion production. Additionally, proinflammatory cytokines were quantified in PF.

Results

PF primed neutrophils via receptor-dependent and receptor-independent mechanisms. There were significant increases in the proinflammatory cytokines interleukin-6 and tumor necrosis factor-¦Á in PF correlating with the development of intra-abdominal sepsis.

Conclusions

PF represents a priming agent for naive polymorphonuclear cells in intra-abdominal sepsis. This may be secondary to increased levels of proinflammatory cytokines. Strategies to reduce the amount of PF may decrease the systemic inflammatory response by reducing a priming agent for neutrophils.