The nitroxide Tempo inhibits hydroxyl radical production from the Fenton-like reaction of iron(II)-citrate with hydrogen peroxide

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• 作者：Fengqiang Shi ; Peifeng Zhang ; Yujia Mao ; Can Wang ; Meiqing Zheng ; Zhongwei Zhao ; ^{zzhao@ccmu.edu.cn}
• 关键词：Nitroxide Tempo ; Iron(II)-citrate complex ; Reactive oxygen species ; Fenton-like reaction ; EPR ; Fluorescence
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2017
• 出版时间：29 January 2017
• 年：2017
• 卷：483
• 期：1
• 页码：159-164
• 全文大小：666 K
• 卷排序：483

文摘

Tempo inhibits OH production from the reaction of Fe(II)-citrate with H2O2 at pH7. Our data show Tempo inhibits OH production from this Fenton-like reaction by 90%. Inhibition is due to Fe(II)-citrate oxidation by Tempo shown for the first time. The oxidation stoichiometry is determined to be Tempo:Fe(III)-citrate = 1.1:1.0. A scheme is proposed to illustrate the roles of nitroxides in Fenton reactions.