Ageing of BIOS for ~ 5 years at 4°C did not influence the composition or proportion of Fe-bearing minerals within BIOS.
Bacterial cells and their polymers likely stabilized 2-line ferrihydrite against transformation.
The rates of microbial Fe(III) reduction of aged BIOS were significantly lower than that of fresh BIOS.
TEM of aged BIOS revealed aggregation of ferrihydrite nanoparticles and possible development of crystallinity.