Data set of optimal parameters for colorimetric red assay of epoxide hydrolase activity

详细信息    查看全文

• 作者：Gabriel Stephani de Oliveira^a ; Patricia Pereira Adriani^a ; Flavia Garcia Borges^a ; Adriana Rios Lopes^b ; Patricia T. Campana^a ; Felipe S. Chambergo^a ; ^{fscha@usp.br" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Colorimetric red assay ; Epoxide hydrolase ; Kinetic parameters
• 刊名：Data in Brief
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：8
• 期：Complete
• 页码：436-440
• 全文大小：1505 K

文摘

The data presented in this article are related to the research article entitled “Epoxide hydrolase of Trichoderma reesei: Biochemical properties and conformational characterization” [1]. Epoxide hydrolases (EHs) are enzymes that catalyze the hydrolysis of epoxides to the corresponding vicinal diols. This article describes the optimal parameters for the colorimetric red assay to determine the enzymatic activity, with an emphasis on the characterization of the kinetic parameters, pH optimum and thermal stability of this enzyme. The effects of reagents that are not resistant to oxidation by sodium periodate on the reactions can generate false positives and interfere with the final results of the red assay.