Effectiveness of EDTA and Modified Salt Solution to Detach and Kill Cells from Enterococcus faecalis Biofilm
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- 作者：Josiane de Almeida ; DDS ; MSc&lowast ; ; &dagger ; ; dealmeidajosiane@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Michel Hoogenkamp ; Ing&lowast ; ; Wilson T. Felippe ; DDS ; MSc ; PhD&dagger ; ; Wim Crielaard ; PhD&lowast ; ; Suzette V. van der Waal ; DDS ; PhD&lowast ; ; &Dagger ;
- 关键词：Biofilm detachment ; biofilm disruption ; disinfection ; EDTA ; Enterococcus faecalis ; modified salt solution ; propidium monoazide ; quantitative polymerase chain reaction
- 刊名：Journal of Endodontics
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：42
- 期：2
- 页码：320-323
- 全文大小：290 K
文摘
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EDTA detached most cells in biofilms.
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A modified salt solution had a great antimicrobial effect and also detached 94% of biofilm cells.
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The treatment of samples with propidium monazide can serve as an alternative method to plating and culturing.