The Protein CTCF Is Required for the Enhancer Blocking Activity of Vertebrate Insulators

详细信息    查看全文

• 作者：Adam C. Bell ; Adam G. West ; Gary Felsenfeld
• 刊名：Cell
• 出版年：1999
• 出版时间：6 August 1999
• 年：1999
• 卷：98
• 期：3
• 页码：387-396
• 全文大小：448 K

文摘

An insulator is a DNA sequence that can act as a barrier to the influences of neighboring cis-acting elements, preventing gene activation, for example, when located between an enhancer and a promoter. We have identified a 42 bp fragment of the chicken β-globin insulator that is both necessary and sufficient for enhancer blocking activity in human cells. We show that this sequence is the binding site for CTCF, a previously identified eleven–zinc finger DNA-binding protein that is highly conserved in vertebrates. CTCF sites are present in all of the vertebrate enhancer-blocking elements we have examined. We suggest that directional enhancer blocking by CTCF is a conserved component of gene regulation in vertebrates.