Hypermethylation of the Keap1 gene inactivates its function, promotes Nrf2 nuclear accumulation, and is involved in arsenite-induced human keratinocyte transformation
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- 作者：Dapeng Wanga ; b ; Yuan Maa ; Xu Yanga ; Xiguo Xua ; Yingying Zhaoa ; Zhen Zhua ; Xiaojuan Wanga ; Hanyi Denga ; Chunchun Lia ; Fenfang Gaoa ; Jian Tonga ; Kenzo Yamanakac ; Yan Ana ; dranyan@126.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：ROS ; reactive oxygen species ; Nrf2 ; nuclear factor E2-related factor 2 ; ARE ; antioxidant response element ; HO-1 ; heme oxygenase 1 ; SOD ; superoxide dismutase ; CAT ; catalase ; Keap1 ; Kelch-like ECH-associated protein 1 ; Bach1 ; BTB and CNC homolog 1 ; FBS ; fetal bovine serum ; MMP-9 ; matrix metalloproteinase-9 ; PBS ; phosphate-buffered saline ; MDA ; malondialdehyde ; 4-HNE ; 4-hydroxynonenal ; LPO ; lipid peroxidation ; TTBS ; Tween 20 Tris-buffered saline ; DNMT ; DNA methyltransferase ; 5-Aza-dC ; 5-aza-2&prime
- 刊名：Free Radical Biology and Medicine
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：89
- 期：Complete
- 页码：209-219
- 全文大小：6536 K
文摘
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Long term arsenite exposure reduces ROS and lipid peroxidation levels.
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Long term arsenite exposure enhances Nrf2-mediated antioxidant levels.
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Long term arsenite exposure induces hypermethylation of Keap1 gene promoter region.
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Long term arsenite exposure down-regulates DNMT-1 but up-regulates DNMT-3 expression.
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Demethylation of Keap1 gene reduces malignant degree of arsenite-transformed cells.