Polycistronic tRNA and CRISPR guide-RNA enables highly efficient multiplexed genome engineering in human cells

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• 作者：Fengping Dong^a ; ¹ ; Kabin Xie^b ; ^c ; ¹ ; Yueying Chen^b ; Yinong Yang^b ; ^yuy3@psu.edu ; Yingwei Mao^a ; ^yzm1@psu.edu
• 关键词：Genome editing ; Polycistronic-tRNA-gRNA ; HDAC ; Wnt
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2017
• 出版时间：22 January 2017
• 年：2017
• 卷：482
• 期：4
• 页码：889-895
• 全文大小：1789 K
• 卷排序：482

文摘

An efficient strategy to produce multiple gRNAs via the intrinsic tRNA-processing system. Success in knockout of multiple HDACs in mammalian cells using PTG system. Demonstration of differential effect of HDACs on the Wnt signaling pathway.