Development of a duplex real-time RT-PCR for the simultaneous detection and differentiation of Theiler’s murine encephalomyelitis virus and rat theilovirus
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- 作者：Wen Yuan ; Jing Wang ; Fengjiao Xu ; Bihong Huang ; Yuexiao Lian ; Dan Rao ; Xueqin Yin ; Miaoli Wu ; Yujun Zhu ; Yu Zhang ; Ren Huang ; Pengju Guo ; guo_pengju@hotmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Theiler&rsquo ; s murine encephalomyelitis virus ; Rat theilovirus ; Duplex real-time RT-PCR ; Differentiation
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：236
- 期：Complete
- 页码：139-146
- 全文大小：2593 K
文摘
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A duplex real-time RT-PCR was developed and evaluated for detection of TMEV and RTV.
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The duplex assay could differentiate between TMEV and RTV.
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The duplex assay was specific, sensitive and reproducible.
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The duplex assay was more sensitive and effective than conventional RT-PCR.
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It is a useful tool for routine health monitoring of laboratory rodents.