Development of an oxidative dehydrogenation-based fluorescent probe for Cu²⁺ and its biological imaging in living cells

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• 作者：Jiangli Fan ; ^{fanjl@dlut.edu.cn} ; Xiaojian Liu ; Mingming Hu ; Hao Zhu ; Fengling Song ; Xiaojun Peng ; ^{pengxj@dlut.edu.cn}
• 关键词：BODIPY ; Fluorescent probe ; Cu2+ ; Oxidative dehydrogenation ; Living cells
• 刊名：Analytica Chimica Acta
• 出版年：2012
• 出版时间：20 July, 2012
• 年：2012
• 卷：735
• 期：Complete
• 页码：107-113
• 全文大小：1188 K

文摘

Based on a boron dipyrromethene (BODIPY) derivative containing an N, O and S tridentate ligand, a Cu²⁺ fluorescent probe BTCu was developed. The detection mechanism was verified as Cu²⁺-promoted oxidative dehydrogenation of an amine moiety, leading to a formation of a fluorescent Cu⁺-Schiff base complex. Free BTCu exhibited a maximum absorption wavelength at 496 nm, and a very weak maximum emission at 511 nm. Upon addition of various metals ions, it showed large fluorescence enhancement toward Cu²⁺ (417-fold in MeCN and 103-fold in MeCN/HEPES solution, respectively) with high selectivity. The detection limits are as low as 1.74 ¡Á 10^? M and 4.96 ¡Á 10^? M in the two different solutions, respectively. And BTCu could work in a wide pH range with an extraordinary low pK_a of 1.21 ¡À 0.06. Using fluorescence microscopy, the probe was shown to be capable of penetrating into living cells and imaging intracellular Cu²⁺ changes.