Multi-nanomaterial electrochemical biosensor based on label-free graphene for detecting cancer biomarkers

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• 作者：Bing Jin ; Ping Wang ; Hongju Mao ; Bing Hu ; Honglian Zhang ; Zule Cheng ; Zhenhua Wu ; Xiaojun Bian ; Chunping Jia ; Fengxiang Jing ; Qinghui Jin ; Jianlong Zhao
• 关键词：Electrochemical biosensor ; Graphene ; Gold nanoparticle ; Magnetic bead ; Cancer markers
• 刊名：Biosensors and Bioelectronics
• 出版年：15 May, 2014
• 年：2014
• 卷：55
• 期：Complete
• 页码：464-469
• 全文大小：1528 K

文摘

Developing a rapid, accurate and sensitive electrochemical biosensor for detecting cancer biomarkers is important for early detection and diagnosis. This work reports an electrochemical biosensor based on a graphene (GR) platform which is made by CVD, combined with magnetic beads (MBs) and enzyme-labeled antibody-gold nanoparticle bioconjugate. MBs coated with capture antibodies (Ab1) were attached to GR sheets by an external magnetic field, to avoid reducing the conductivity of graphene. Sensitivity was also enhanced by modifying the gold nanoparticles (AuNPs) with horseradish peroxidase (HRP) and the detection antibody (Ab2), to form the conjugate Ab2-AuNPs-HRP. Electron transport between the electrode and analyte target was accelerated by the multi-nanomaterial, and the limit of detection (LOD) for carcinoembryonic antigen (CEA) reached 5 ng mL^鈭?. The multi-nanomaterial electrode GR/MBs-Ab1/CEA/Ab2-AuNPs-HRP can be used to detect biomolecules such as CEA. The EC biosensor is sensitive and specific, and has potential in the detection of disease markers.