T lineage differentiation from induced pluripotent stem cells

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• 作者：Fengyang Lei ; Rizwanul Haque ; Lauren Weiler ; Kent E. Vrana ; Jianxun Song
• 关键词：T cells ; Induced pluripotent stem cells ; Notch signaling ; Differentiation
• 刊名：Cellular Immunology
• 出版年：2009
• 出版时间：2009
• 年：2009
• 卷：260
• 期：1
• 页码：1-5
• 全文大小：736 K

文摘

Induced pluripotent stem (iPS) cells have potential to differentiate into T lymphocytes, however, the actual ability of iPS cells to develop into T lineages is not clear. In this study, we co-cultured iPS cells on OP9 cells expressing the Notch ligand Delta-like 1 (DL1), the iPS cells differentiated into T lymphocytes. In addition, in vitro stimulation of iPS cell-derived T lymphocytes resulted in secretion of IL-2 and IFN-γ. Moreover, adoptive transfer of iPS cell-derived T lymphocytes into Rag-deficient mice reconstituted their T cell pools. These results indicate that iPS cells are able to follow the normal program of T cell differentiation.