Astragalin suppresses inflammatory responses via down-regulation of NF-¦ÊB signaling pathway in lipopolysaccharide-induced mastitis in a murine model

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• 作者：Fengyang Li ; Dejie Liang ; Zhengtao Yang ; Tiancheng Wang ; Wei Wang ; Xiaojing Song ; Mengyao Guo ; Ershun Zhou ; Depeng Li ; Yongguo Cao ; Naisheng Zhang
• 关键词：Astragalin ; Lipopolysaccharide (LPS) ; Mastitis ; Cytokine ; Nuclear factor-kappaB (NF-¦ÊB)
• 刊名：International Immunopharmacology
• 出版年：2013
• 出版时间：October, 2013
• 年：2013
• 卷：17
• 期：2
• 页码：478-482
• 全文大小：1256 K

文摘

Mastitis is a prevalent and economic disease around the world and defined as infection and inflammation of the mammary gland. Astragalin, a bioactive component isolated from persimmon or Rosa agrestis, has been reported to have anti-inflammatory properties. To investigate the potential therapeutic effect of astragalin in mastitis, a murine model of mastitis was induced by administration of LPS in mammary gland. Astragalin was applied 1 h before and 12 h after LPS treatment. The results showed that astragalin attenuated the infiltration of inflammatory cells, the activity of myeloperoxidase (MPO) and the expression of tumor necrosis factor-¦Á (TNF-¦Á), interleukin-6 (IL-6) and interleukin-1¦Â (IL-1¦Â) in a dose-dependent manner. Additionally, Western blotting results showed that astragalin efficiently blunt decreased nuclear factor-kappaB (NF-¦ÊB) activation by inhibiting the degradation and phosphorylation of I¦ÊB¦Á and the nuclear translocation of p65. These results suggested that astragalin exerts anti-inflammatory properties in LPS-mediated mastitis, possibly through inhibiting inhibition of the NF-¦ÊB signaling pathway, which mediates the expression of pro-inflammatory cytokines. Astragalin may be a potential therapeutic agent against mastitis.