Horseradish peroxidase (r1HRP) was reconstituted onto C11-alkanethiol-conjugated hemin.
R1HRP was self-assembled on Au nanoparticle (NP) electrodes via the exposed thiol tail.
Electron transfer between r1HRP and electrode is enhanced when proceeding via the Au NP relay.
Demonstrated NP-biohybrid system allows advanced programmable design of bioelectronics interfaces.