Mature Pseudomonas putida mono-species biofilms were cultured under hydrodynamic conditions.
Short and long term impacts of Ag NPs on biofilm morphology, viability and activity were studied.
Dose dependent toxicity patterns were reported post exposure to 0.01–100 mg L−1.
Recovering biofilms were characterised 24 h post exposure.
Dynamic biofilm based models offer enhanced nanosafety assessment.