CCR2 Deficiency Results in Increased Osteolysis in Experimental Periapical Lesions in Mice

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• 作者：Thiago Pompermaier Garlet ; DDS ; MS^&lowast ; ; Sandra Yasuyo Fukada ; DDS ; PhD^&lowast ; ; Isabella Francisco Saconato ; PharmD^&lowast ; ; Mario Julio Avila-Campos ; PhD^&dagger ; ; Tarcí ; lia Aparecida da Silva ; DDS ; PhD^&Dagger ; ; Gustavo Pompermaier Garlet ; DDS ; PhD^§ ; ; Fernando de Queiroz Cunha ; PhD^&lowast ; ; ^{fdqcunha@fmrp.usp.br" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Bone resorption ; CCR2 ; chemokine receptors ; OPG ; periapical lesions ; RANKL
• 刊名：Journal of Endodontics
• 出版年：2010
• 出版时间：February 2010
• 年：2010
• 卷：36
• 期：2
• 页码：244-250
• 全文大小：995 K

文摘

Periapical lesions are chronic inflammatory disorders of periradicular tissues caused by etiologic agents of endodontic origin. The inflammatory chemokines are thought to be involved in the latter observed osteolysis. With a murine model of experimental periapical lesion, the objective of this study was to evaluate the role of the chemokine receptor CCR2 in the lesion progression, osteoclast differentiation and activation, and expression of inflammatory osteolysis-related mediators.

Methods

For lesion induction, right mandibular first molars were opened surgically with a ¼ carbine bur, and 4 bacterial strains were inoculated in the exposed dental pulp; left mandibular first molars were used as controls. Animals were killed at 3, 7, 14, and 21 days after surgeries to evaluate the kinetics of lesion development.

Results

CCR2 KO mice showed wider lesions than WT mice. CCR2 KO mice also expressed higher levels of the osteoclastogenic and osteolytic factors, receptor activator of nuclear factor kappa B ligand (RANKL) and cathepsin K, of the proinflammatory cytokine tumor necrosis factor–alpha, and of the neutrophil migration related chemokine, KC.

Conclusions

These results suggest that CCR2 is important in host protection to periapical osteolysis.