A simultaneous analytical method was developed to discriminate Aloe arborescens, Aloe barbadensis and Aloe ferox.
HPLC–MS/MS and HPLC-DAD were applied to qualitative and quantitative determination of eight maker compounds.
The extraction conditions were optimized by response surface methodology.
A partial least squares-discriminant analysis was used to identify the three aloe species.
Characteristic compounds for identification of aloe species were revealed between three aloes.