Overexpression of four and a half LIM domains protein 2 promotes epithelial-mesenchymal transition-like phenotype in fish pre-osteoblasts

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• 作者：Marta S. Rafael^a ; Vincent Laizé ; ^a ; ^{vlaize@ualg.pt} ; Clá ; udia Florindo^b ; ^c ; Serena Ferraresso^d ; Luca Bargelloni^d ; M. Leonor Cancela^a ; ^b ; ^{lcancela@ualg.pt}
• 关键词：Four and a half LIM domains protein 2 (FHL2) ; Fish bone-derived cell line (VSa16) ; Epithelial-mesenchymal transition (EMT) ; Microarray analysis
• 刊名：Biochimie
• 出版年：2012
• 出版时间：May, 2012
• 年：2012
• 卷：94
• 期：5
• 页码：1128-1134
• 全文大小：905 K

文摘

FHL2 is a multifunctional protein involved in gene transcription regulation and cytoarchitecture modulation that has been recently associated with epithelial-mesenchymal transition (EMT) in colon cancer. Overexpression of FHL2 in a fish pre-osteoblastic cell line promoted cell dedifferentiation and impaired its extracellular matrix mineralization capacity. Cell cultures also acquired a novel three-dimensional structure organization, their proliferation rate was enhanced and gene expression profile was altered in agreement with an EMT-like phenotype upon overexpression of FHL2. Altogether, our results provide additional support to the relevance of FHL2 for cell differentiation and its association with hallmarks of cancer phenotype.