The importance of the benzoic acid carboxylate moiety for substrate recognition by CYP199A4 from Rhodopseudomonas palustris HaA2
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- 作者：Tom Colemana ; Rebecca R. Chaoa ; James J. De Vossb ; Stephen G. Bella ; stephen.bell@adelaide.edu.au" class="auth_mail" title="E-mail the corresponding author
- 关键词：BSTFA ; N ; O-bis(trimethylsilyl)trifluoroacetamide ; CYP ; cytochrome P450 enzyme ; DMSO ; dimethyl sulfoxide ; EMM ; E. coli minimal media ; EtOH ; ethanol ; GC ; gas chromatography ; HaPuR ; ferredoxin reductase electron transfer partner of CYP199A4 ; HaPux ; [2Fe&ndash ; 2S] ferredoxin electron transfer partner of CYP199A4 ; HPLC ; high performance liquid chromatography ; IPTG ; Isopropyl β-d-1-thiogalactopyranoside ; LB ; Luria-Bertani broth ; MS ; mass spectrometry ; NADH ; reduced nicotinamide adenine dinucleotide ; T
- 刊名：Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：1864
- 期：6
- 页码：667-675
- 全文大小：1129 K
文摘
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CYP199A4 has high selectivity for the carboxylate group of 4-methoxybenzoic acid.
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This selectivity arises from interactions with arginine and serine residues.
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Alternative functional groups can bind to CYP199A4 but with low affinity.
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The activities of the in vitro enzyme assays can be extrapolated to whole-cell oxidation systems.
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The high regioselectivity for oxidation at the para-substituent is maintained.