IL-5 and IL-17A are critical for the chronic IgE response and differentiation of long-lived antibody-secreting cells in inflamed tissues

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• 作者：Lidiane Zito Grund ; Evilin Naname Komegae ; Mô ; nica Lopes-Ferreira ; Carla Lima ; ^{carlalima@butantan.gov.br}
• 关键词：Fish venom ; IL-5/IL-17A ; Memory B cell ; Long-lived antibody-secreting cells ; IgE
• 刊名：Cytokine
• 出版年：2012
• 出版时间：August, 2012
• 年：2012
• 卷：59
• 期：2
• 页码：335-351
• 全文大小：2047 K

文摘

Prolonged survival of long-lived antibody-secreting cells in the BM has been implicated as a key component of long-term humoral immunity. The current study was designed to uncover the extrinsic signals required for the generation and maintenance of ASC in several niches (peritoneum, spleen and bone-marrow). Our results show that protein mixture of the m>Thalassophryne nattererim> venom induced a chronic Th2 humoral response that is characterized by splenic hyperplasia with GC formation and venom retention by follicular DCs. Retention of B1a in the BM were observed. In the late phase (120 d) of chronic venom-response the largest pool of ASC into the peritoneal cavity consisted of B220^negCD43^high phenotype; the largest pool of ASC into spleen was constituted by B220 positive cells (B220^high and B220^low), whereas the largest pool of ASC into in the BM was constituted by the B220^highCD43^low phenotype; and finally, terminally differentiated cells (B220^negCD43^high) were only maintained in the inflamed peritoneal cavity in late phase. After 120 d a sustained production of cytokines (KC, IL-5, TNF-¦Á, IL-6, IL-17A and IL-23) and leukocytes recruitment (eosinophils, mast cells, and neutrophils) were induced. IL-5- and IL-17A-producing CD4+ CD44+ CD40L+ Ly6C+ effector memory T cells were also observed in peritoneal cavity. Finally, treatment of venom-mice with anti-IL-5- and anti-IL17A-neutralizing mAbs abolished the synthesis of specific IgE, without modifying the splenic hyperplasia or GC formation. In addition, IL-5 and IL-17A negatively regulated the expansion of B1a in peritoneal cavity and BM, and promoted the differentiation of these cells in spleen. And more, IL-5 and IL-17A are sufficient for the generation of ASC B220^neg in the peritoneal cavity and negatively regulate the number of ASC B220^pos, confirming that the hierarchical process of ASC differentiation triggered by venom needs the signal derived from IL-5 and IL-17A.