Radical scavenging activity and cytotoxicity of active quinic acid derivatives from Scorzonera divaricata roots

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• 作者：Yong-Jin Yang^a ; Xu Liu^b ; Hong-Ru Wu^a ; Xiao-Feng He^a ; Yu-Rong Bi^b ; Ying Zhu^a ; ^{zhuy@lzu.edu.cn} ; Zhen-Ling Liu^a
• 关键词：Scorzonera divaricata ; Quinic acid derivatives ; Cytotoxicity ; Radical scavenging capacity
• 刊名：Food Chemistry
• 出版年：2013
• 出版时间：1 June, 2013
• 年：2013
• 卷：138
• 期：2-3
• 页码：2057-2063
• 全文大小：387 K

文摘

Five new quinic acid derivatives and two known 3-O-feruloylquinic acids were isolated from the roots of Scorzonera divaricata Turcz. The new compounds were elucidated as (-)-1,4-di-O-feruloyl-3-O-dihydrocaffeoylquinic acid, (-)-1-O-feruloyl-4-O- dihydrocaffeoylquinic acid, (-)-3,5-di-O-feruloylquinic acid, (-)-1-O-feruloyl-3-O-dihydro- caffeoylquinic acid, and (-)-1-O-feruloyl-5-O-dihydrocaffeoylquinic acid based on extensive spectroscopic studies, including one- and two-dimensional NMR, HRESIMS, UV, and IR results. Five compounds were assessed for antioxidant activity by ABTS and DPPH radical-scavenging assays and for their cytotoxicity against HL-60 and Hep-G2 cell lines by the MTT assay. Three quinic acid derivatives exhibited strong antioxidant activity, with IC₅₀ values of 3.95, 5.87, and 7.45 ¦Ìg/mL against ABTS⁺ and 11.7, 13.6, and 50.1 ¦Ìg/mL against DPPH. (-)-1,4-Di-O-feruloyl-3-O-dihydrocaffeoylquinic acid also exhibited moderate activity against Hep-G2 cell lines with an IC₅₀ value of 14.6 ¦Ìg/mL.