Enzymatic degradation of poly(l-lactide) nanoparticles followed by the release of octenidine and their bactericidal effects
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- 作者：Grit Baier ; Alex Cavallaro ; Kathrin Friedemann ; Beate M眉ller ; Gunnar Glasser ; Krasimir Vasilev ; Katharina L ; fester
- 关键词：Poly(l-lactide) nanoparticles ; Octenidine ; Bactericidal effects
- 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
- 出版年：January, 2014
- 年：2014
- 卷：10
- 期：1
- 页码：131-139
- 全文大小：2113 K
文摘
The enzyme-triggered release of the antimicrobial agent octenidine out of poly(l-lactide)-based nanoparticles (PLLA-NPs) and their in vitro antibacterial activities in the presence of gram-positive and gram-negative bacteria are presented. The formation of the nanoparticles was achieved using a combination of the solvent evaporation and the miniemulsion approach. For the stabilization of the polymeric nanoparticles, non-ionic polymers (polyvinylalcohol [PVA], hydroxyethyl starch [HES], human serum albumin [HSA]) were successfully used for enzymatic degradation; ionic surfactants such as sodium dodecyl sulfate and cetyltrimethylammonium chloride inhibited the enzymatic degradation. The change in pH, size, size distribution and morphology during the degradation process of PLLA-NPs and the release of the antimicrobial agent was studied. The influence of the different amounts of octenidine and of the different stabilizers on the NPs' stability, size, size distribution, morphology, zeta potential and on the surface group's density is discussed. Fluorescently labeled HES-stabilized PLLA-NPs are immobilized by colloidal electrospinning. The observed data from HPLC measurements show that octenidine is released out of PLLA-NPs which are stabilized with PVA, HES or HSA. In bacteria tests the PLLA nanoparticles showed a greater ability to inhibit the growth of Staphylococcus aureus compared to Escherichia coli.
From the Clinical Editor
This article discusses the enzyme-triggered release and antibacterial effects of octenidine from poly(l-lactide)-based nanoparticles demonstrating the viability of this approach for potential future antibacterial therapy.