Silver-graphene oxide nanocomposites as redox probes for electrochemical determination of ¦Á-1-fetoprotein

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• 作者：Yongmei Wu ; Wenju Xu ; ^{xwju@swu.edu.cn} ; Yan Wang ; Yali Yuan ; Ruo Yuan
• 关键词：Silver&ndash ; graphene oxide ; Redox probe ; Label-free ; Electrochemical immunosensor
• 刊名：Electrochimica Acta
• 出版年：2013
• 出版时间：15 January, 2013
• 年：2013
• 卷：88
• 期：Complete
• 页码：135-140
• 全文大小：1137 K

文摘

Herein, a strategy was put forward for sensitive label-free electrochemical immunosensor by using silver-graphene oxide (Ag-GO) as redox probe. Initially, a simple, soft reaction conditions and efficient route was designed to in situ reduce silver nanoparticles (AgNPs) onto graphene oxide (GO) by glucose as reducing agent. The obtained nanocomposite gave a pair of well-defined redox peaks with the advantages of good biocompatibility and chemical stability. Furthermore, the GO provided large surface area for assembly of abundant AgNPs with redox activity, which potentially paved sensitive way for immunodetection. Thus, the Ag-GO nanocomposites were used as redox probe to fabricate a sensitive label-free immunosensor for ¦Á-1-fetoprotein (AFP). In addition, gold nanoparticles (AuNPs) for immobilization of antibody (anti-AFP) could amplify the electrochemical signal and further enhance the sensitivity of the immunosensor due to its excellent conductivity and large surface area. Under the optimal condition, the anodic peak current linearly responded to the logarithm of AFP concentration in a wide range from 0.01 to 100 ng mL^?1 with a low limit of detection of 3 pg mL^?1 (S/N = 3). The designed immunosensor displayed the advantages of simple preparation, high sensitivity, good selectivity and satisfactory stability.