Enhancement of Labeled Alpha-fetoprotein Antibodies and Antigen-antibody Complexes Fluorescence with Silver Nanocolloids

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• 作者：Svetlana Vaschenko^a ; Andrey Ramanenka^a ; ^{a.ramanenka@ifanbel.bas-net.by" class="auth_mail" title="E-mail the corresponding author} ; Olga Kulakovich^a ; Alina Muravitskaya^a ; Dmitry Guzatov^b ; Anatoly Lunevich^c ; Yuri Glukhov^c ; Sergey Gaponenko^d
• 关键词：plasmon-enhanced fluorescence ; silver nanoparticles ; immunoassay ; alpha-fetoprotein ; poin-of-care testing
• 刊名：Procedia Engineering
• 出版年：2016
• 出版时间：2016
• 年：2016
• 卷：140
• 期：Complete
• 页码：57-66
• 全文大小：340 K

文摘

A model immunoassay test system is proposed based on metal enhanced photoluminescence to analyze low concentrations of alpha-fetoprotein, a tumor marker. Antigen-antibody reaction was performed on polystyrene plates coated with silver nanoparticles to increase sensitivity of fluorescent immunoassay and signal-to-noise ratio as compared to silver-free system. The proposed test system model uses layer-by-layer assembly approach, LED excitation and nanowatt photodetection set-up, so it is characterized by smaller probe volume, fast analysis and simplicity. The proposed model system offers alpha-fetoprotein detection at concentrations used in clinical practice. Photostability and photoluminescence enhancement for labeled alpha-fetoprotein antibodies on a silver substrate were found to depend on antibodies concentration. Labeled antibodies photostability on substrates was found to deviate within 20% as compared to silver-free samples whereas photoluminescence intensity enhancement is typically in the range of 6-8 times.