Valeric acid supplementation combined to mild hypothermia increases productivity in CHO cell cultivations
详细信息 查看全文
- 作者：Juliana Coronela ; jcl@peq.coppe.ufrj.br" class="auth_mail" title="E-mail the corresponding author ; lecc@peq.coppe.ufrj.br" class="auth_mail" title="E-mail the corresponding author ; Sandra Klausingb ; sandra.klausing@xell.ag" class="auth_mail" title="E-mail the corresponding author ; Christoph Heinrichb ; christoph.heinrich@xell.ag" class="auth_mail" title="E-mail the corresponding author ; Thomas Nollc ; thomas.noll@uni-bielefeld.de" class="auth_mail" title="E-mail the corresponding author ; Alvio Figueredo-Carderod ; 1 ; alvio.figueredo@bio.fiocruz.br" class="auth_mail" title="E-mail the corresponding author ; Leda R. Castilhoa ; leda@peq.coppe.ufrj.br" class="auth_mail" title="E-mail the corresponding author
- 关键词：CHO cell culture ; Cultivation temperature ; Butyrate and valerate supplementation ; Valeric acid and mild hypothermia ; Cell viability and product titre ; Cell specific productivity (qp)
- 刊名：Biochemical Engineering Journal
- 出版年：2016
- 出版时间：15 October 2016
- 年：2016
- 卷：114
- 期：Complete
- 页码：101-109
- 全文大小：656 K
文摘
- ass="listitem" id="list_lis0005">
- ass="label">
ar0005">Valeric and butyric acid supplementation increased qp under mild hypothermia.
- ass="label">
ar0010">Supplementation with 1 mM valeric acid at 31 °C led to the highest productivity.
- ass="label">
ar0015">Valeric acid was less cytotoxic than butyric acid.
- ass="label">
ar0020">Percentage of cells in G0 + G1 phase was higher at 31 °C, mainly with valeric acid.
- ass="label">
ar0025">The proliferation state of cells [S/(G0 + G1) ratio] inversely correlated with qp.