Antimicrobial activity of Amazonian oils against Paenibacillus species

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• 作者：Roberto Christ Vianna Santos^a ; ^{robertochrist@gmail.com} ; ^{roberto@unifra.br} ; Camilla Filippi dos Santos Alves^a ; Taiane Schneider^a ; Leonardo Quintana Soares Lopes^a ; Carlos Aurich^b ; Janice Luehring Giongo^b ; Adriano Brandelli^c ; Rodrigo de Almeida Vaucher^a ; ^b
• 关键词：Andiroba ; Copaí ; ba ; Apis mellifera ; Paenibacillus larvae
• 刊名：Journal of Invertebrate Pathology
• 出版年：2012
• 出版时间：March, 2012
• 年：2012
• 卷：109
• 期：3
• 页码：265-268
• 全文大小：290 K

文摘

The Gram-positive, spore-forming bacterium Paenibacillus larvae is the primary bacterial pathogen of honeybee brood and the causative agent of American foulbrood disease (AFB). One of the feasible alternative treatments being used for their control of this disease is essential oils. In this study in vitro antimicrobial activity of Andiroba and Copa¨ªba essential oils against Paenibacillus species, including P. larvae was evaluated. Minimal inhibitory concentration (MIC) in Mueller-Hinton broth by the microdilution method was assessed. Andiroba registered MIC values of 1.56-25 % , while the MICs values obtained for Copa¨ªba oil were of 1.56-12.5 % . In order to determine the time-response effect of essential oils on P. larvae, this microorganism was exposed to the oils for up to 48 h. After 24 h treatment with Andiroba oil and after 48 h treatment with Copa¨ªba oil no viable cells of P. larvae ATCC 9545 were observed. The possible toxic effect of essential oils were assessed by the spraying application method of the same concentrations of MICs. Bee mortality was evident only in treatment with Andiroba oil and the Copa¨ªba oil shows no toxic effects after 10 days of observation. Taking together ours results showed for the first time that these oils presented a high activity against Paenibacillus species showing that Copa¨ªba oil may be a candidate for the treatment or prevention of AFB.