Genome analysis of Chlamydia trachomatis for functional characterization of hypothetical proteins to discover novel drug targets

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• 作者：Ahmad Abu Turab Naqvi^a ; ¹ ; Safikur Rahman^b ; ¹ ; Rubi^a ; Firdaus Zeya^c ; Kundan Kumar^a ; Hani Choudhary^d ; Mohammad Sarwar Jamal^e ; Jihoe Kim^b ; ^{kimjihoe@ynu.ac.kr} ; Md. Imtaiyaz Hassan^a ; ^{mihassan@jmi.ac.in}
• 关键词：Hypothetical proteins ; Virulence factors ; Drug discovery
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：96
• 期：Complete
• 页码：234-240
• 全文大小：819 K
• 卷排序：96

文摘

C. trachomatis is a Gram-negative bacterium that causes trachoma and sexually transmitted disease (STD) Chlamydia in humans. Chlamydial genital infections are the most frequent among all communicable diseases. The D/UW-3/Cx strain of C. trachomatis contains 935 genes and three pseudogenes. Out of these genes, 887 genes code for proteins while six for rRNA, 37 tRNA, and three genes translate into other RNAs. The proteome of C. trachomatis made of 887 proteins contains 269 Hypothetical proteins (HPs) that are subjected to functional characterization. This study suggests some known methods of functional characterization of such HPs. All of these methods are explicitly used to assign functions to the HPs with the accuracy of more than 90%. After extensive analysis of all the HPs, we have successfully assigned functions to 89 HPs with high precision. In the newly assigned HPs, there are enzymes, transporters, binding proteins, proteins involved in biosynthesis and regulatory processes and proteins with miscellaneous functions. The study suggests that the functionally annotated HPs may play a vital role in the growth and pathogenesis of this organism. Therefore, they can be considered potential drug targets.