Detection of the reemerging agent Burkholderia mallei in a recent outbreak of glanders in the United Arab Emirates by a newly developed fliP-based polymerase chain reaction assay
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- 作者：Holger C. Scholz ; Marina Joseph ; Herbert Tomaso ; Sascha Al Dahouk ; Angela Witte ; Joerg Kinne ; Ralph M. Hagen ; Renate Wernery ; Ulrich Wernery and Heinrich Neubauer
- 关键词：Burkholderia mallei ; Glanders ; PCR assay ; Flagellin P
- 刊名：Diagnostic Microbiology and Infectious Disease
- 出版年：2006
- 出版时间：April 2006
- 年：2006
- 卷：54
- 期：4
- 页码：241-247
- 全文大小：208 K
文摘
A polymerase chain reaction (PCR) assay targeting the flagellin P (fliP)-I S407A genomic region of Burkholderia mallei was developed for the specific detection of this organism in pure cultures and clinical samples from a recent outbreak of equine glanders. Primers deduced from the known fliP-IS407A sequence of B. mallei American Type Culture Collection (ATCC) 23344T allowed the specific amplification of a 989-bp fragment from each of the 20 B. mallei strains investigated, whereas other closely related organisms tested negative. The detection limit of the assay was 10 fg for purified DNA of B. mallei ATCC 23344T. B. mallei DNA was also amplified from various tissues of horses with a generalized B. mallei infection. The developed PCR assay can be used as a simple and rapid tool for the specific and sensitive detection of B. mallei in clinical samples.