Functional replacement of yeast flavocytochrome b₂ with bacterial l-lactate dehydrogenase

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• 作者：Hiroaki  ; Sakai¹ ; Kazuhiro  ; Nagahama¹ ; Hisataka  ; Taguchi¹ ; Takashi  ; Akamatsu¹ ; Shigeru  ; Morimura² ; Kenji  ; Kida² ; Masayoshi  ; Matsuoka¹ ;   ; ^{matsuoka@bio.sojo-u.ac.jp}
• 关键词： ; small-caps"">l-Lactate dehydrogenase ; Chimera ; Genetic complementation ; Matrix targeting ; Mitochondrial electron transport
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2010
• 出版时间：September 2010
• 年：2010
• 卷：110
• 期：3
• 页码：269-272
• 全文大小：232 K

文摘

Using yeast genetic complementation, we show here that Rhodobacter sphaeroides l-lactate dehydrogenase can functionally replace flavocytochrome b₂ in Saccharomyces cerevisiae, only when a matrix-targeting signal of flavocytochrome b₂ is fused with the bacterial enzyme. The recombinant l-lactate dehydrogenase may add alternative route of mitochondrial electron transport other than flavocytochrome b₂.