Single vs multiple-spore inoculum effect on growth kinetic parameters and modeled probabilities of growth and aflatoxin B1 production of Aspergillus flavus on pistachio extract agar
Differences in growth and AFB1 production of A. flavus at two inoculation levels were assessed. Conditions were set in order to illustrate a possible transport/storage contamination scenario. Significant differences were found when changing from the multiple spore to the single spore inoculation. Predicted probabilities were lower when colonies came up from single spores. The number of spores used in predictive mycology should be carefully controlled.