Evaluation of Antinociceptive Activity of Methanol Extract from Cleome rutidosperma in Mice

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• 作者：Prawej Ansari^a ; ^b ; ^{chemist89ansari@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Mitali Debnath^a ; Md. Foyez Ahmad^a ; Shofiul Azam^a ; ^b ; Shafia Akther^a ; Gazi Md. Mustakim^a ; Md. Hamza Naquib^a ; Shammy Sarwar^c
• 关键词：anti-nociceptive ; Cleome rutidosperma ; hot-plate method ; tail-flick tests
• 刊名：Chinese Herbal Medicines
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：8
• 期：3
• 页码：273-279
• 全文大小：714 K

文摘

Cleome rutidosperma (Capparidaceae), commonly known as “Fringed Spider Flower”, is a medicinal plant found in Southeast Asia. C. rutidosperma is used in folk medicine for diuretic, laxative, analgesic, anti-inflammatory, antipyretic, antimicrobial, anti-oxidant, hypoglycemic, and anthelmintic activities. We have evaluated the anti-nociceptive properties of methanol extract from C. rutidosperma (MECR) in vivo.

Methods

Thermal method (hot plate test and tail flick test) was induced to judge the anti-inflammatory effect and couple of chemical method also used (formalin induced licking test; writhing test carried by acetic acid) to evaluate analgesic effect. Both of these tests were made over animal models, like mice and rats. Two different doses (100 and 200 mg/kg) were used for each case of test, while morphine sulphate (5mg/kg, ip) was used as reference drug.

Results

MECR demonstrated the significantly anti-nociceptive activity in the analgesic and anti-inflammatory tests by reducing nociception in mice models (P < 0.001). In the hot-plate and tail-flick tests, MECR significantly elongated the time to response to the thermal stimuli (100 and 200 mg/kg with P < 0.05, 0.001). The remarkable increase in the latency was observed at 90 and 120 min. In acetic acid-induced writhing test and formalin induced licking test for anti-inflammatory activity, MECR at 100 and 200 mg/kg doses exhibited significant (P < 0.001) reduction of writhing and licking response.

Conclusion

The anti-inflammatory and analgesic effects of C. rutidosperma propose that this effect may be a result of both peripheral and central mechanisms. Further study is required to ensure the proper mechanism of action as well as the active ingredient.