Characterization of an HIV-1 p24^gag epitope recognized by a CD8⁺ cytotoxic T-cell clone

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• 作者：Buseyne ; Florence ; Stevanovic ; Stefan ; Rammensee ; Hans-Georg ; Rivié ; re ; Yves
• 关键词：Cytotoxic T-lymphocyte ; CTL epitope ; HLA-C
• 刊名：Immunology Letters
• 出版年：1997
• 出版时间：March, 1997
• 年：1997
• 卷：55
• 期：3
• 页码：145-149
• 全文大小：428 K

文摘

A CD8⁺ cytotoxic T-cell clone that recognized HIV p24^gag was isolated from an infected individual. The minimal epitope was localized to amino acids 308-316 (QASQEVKNW). Using allogeneic target cells, we found that lysis was restricted by the HLA-Cw0401 molecule. We observed that C1R cells, that express the HLA-Cw0401 allele are able to present the peptide to the cytotoxic clone, but with reduced efficiency. Other B-cell lines, that have been genotyped as HLA-Cw0401⁺ were unable to present the peptide to the clone, suggesting the existence of other variants of HLA-Cw0401 or a loss of cell surface expression of this molecule.