Corrigendum to “Cross-clade-specific cytotoxic T lymphocytes in HIV-1-infected children” [Virology 250 (1998) 316324]
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- 作者：Florence Buseyne ; Marie Laure Chaix ; Bé ; atrice Fleury ; Olivier Manigart ; Marianne Burgard ; Sté ; phane Blanche ; Christine Rouzioux ; Yves Riviè ; re
- 关键词：HIV-1 ; gp41 ; Neutralizing antibody ; MPER
- 刊名：Virology
- 出版年：2008
- 出版时间：15 March 2008
- 年：2008
- 卷：372
- 期：2
- 页码：457
- 全文大小：69 K
文摘
Human immunodeficiency virus type 1 (HIV-1) transmembrane glycoprotein gp41 is targeted by broadly-reactive neutralizing antibodies 2F5 and 4E10, making it an attractive target for vaccine development. To better assess immunogenic properties of gp41, we generated five soluble glutathione S-transferase fusion proteins encompassing C-terminal 30, 64, 100, 142, or 172 (full-length) amino acids of gp41 ectodomain from M group consensus envelope sequence. Antibody responses in HIV-1-infected patients were evaluated using these proteins and overlapping peptides. We found (i) antibody responses against different regions of gp41 varied tremendously among individual patients, (ii) patients with stronger antibody responses against membrane-proximal external region exhibit broader and more potent neutralizing activity, and (iii) several patients mounted antibodies against epitopes that are near, or overlap with, those targeted by 2F5 or 4E10. These soluble gp41 fusion proteins could be an important source of antigens for future vaccine development efforts.