Dual Function of a Human Immunodeficiency Virus (HIV)-Specific Cytotoxic T-Lymphocyte Clone: Inhibition of HIV Replication by Noncytolytic Mechanisms and Lysis of HIV-Infected CD4+ Cells

详细信息    查看全文

• 作者：Buseyne ; Florence ; Fé ; vrier ; Michè ; le ; Garcia ; Sylvie ; Gougeon ; Marie Lise ; Riviè ; re ; Yves
• 刊名：Virology
• 出版年：1996
• 出版时间：November 1, 1996
• 年：1996
• 卷：225
• 期：1
• 页码：248-253
• 全文大小：123 K

文摘

CD8+ T cells may play a beneficial role in human immunodeficiency virus (HIV)-infected patients by two mechanisms: HIV-specific cytotoxic activity and secretion of a soluble mediator(s) that inhibits HIV replicationin vitro.Here we characterized both activities mediated by an HIV p24^gag-specific cytotoxic T lymphocyte (CTL) CD8+ clone derived from an HIV-infected patient. When the CTL clone was mixed with HIV-infected autologous CD4+ T cells, viral replication was suppressed. This viral inhibition was observed in heterologous CD4+ T cells and when CD8+ and CD4+ populations were separated by a semipermeable membrane, demonstrating the involvement of a diffusible factor(s). The lysis of autologous HIV-infected T cells was also detected. However, HIV suppression was more efficient when CD4+ and CD8+ T cells shared major histocompatibility complex alleles and were in direct contact. Thus, one and the same CD8+ T cell population can mediate both lysis of HIV-infected targets and nonlytic suppression of HIV replication. These results underline the multiple roles of CD8+ T lymphocytes in the suppression of HIV-infected cells.