Cancer cell motility is affected through 3D cell culturing and SCF/c-Kit pathway but not by X-irradiation

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• 作者：Fabian Eberle^a ; Miriam F. Saulich^a ; Florian H. Leinberger^a ; Werner Seeger^b ; Rita Engenhart-Cabillic^a ; ^c ; Ekkehard Dikomey^a ; ^d ; Jö ; rg H&auml ; nze^e ; Katja Hattar^f ; Florentine S.B. Subtil^a ; ^c ; ^{Florentine.Subtil@staff.uni-marburg.de" class="auth_mail" title="E-mail the corresponding author}
• 关键词：X-irradiation ; Cancer cell motility ; 3D cell culture ; SCF/c-Kit pathway ; siRNA
• 刊名：Radiotherapy and Oncology
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：119
• 期：3
• 页码：537-543
• 全文大小：1680 K

文摘

Success of radiotherapy is often limited by therapy resistance and metastasis resulting from cancer cell motility. It was tested in vitro whether this cancer cell motility is affected by growth condition, active SCF/c-Kit pathway or X-irradiation.

Materials and methods

sp0010">Cell motility was measured with BioCoat™ Matrigel™ invasion chamber using four different cancer cell lines (NSCLC: H23, H520, H226 and PrCa: DU145). Cells were grown in 2D or 3D, SCF was knocked down by siRNA and cells were irradiated with 2 or 6 Gy.

Results

sp0015">All cell lines except H520 showed a 2–3-fold increase in cell motility when grown in 3D. This effect was considered to result from the EMT-like change seen when cells were grown in 3D as indicated by the enhanced expression of vimentin and N-cadherin and reduction of E-cadherin. Just the opposite trends were found for H520 cells. Knockdown of SCF was found to result in reduced cell motility for both 2D and 3D. In contrast, X-irradiation did not modulate cell motility neither under 2D nor 3D. In line with this, X-irradiation did neither induce the expression of EMT-associated genes nor SCF.

Conclusion

sp0020">X-irradiation affects neither the expression of important EMT genes such as vimentin, E-cadherin and N-cadherin nor SCF/c-Kit signaling and, as a consequence, does not alter cell motility.