A self-assembling fluorescent dipeptide conjugate for cell labelling

详细信息    查看全文

• 作者：Steven Kirkham^a ; Ian W. Hamley^a ; ^{I.W.Hamley@reading.ac.uk" class="auth_mail" title="E-mail the corresponding author} ; Andrew M. Smith^a ; ¹ ; Ricardo M. Gouveia^b ; Che J. Connon^b ; Mehedi Reza^c ; Janne Ruokolainen^c
• 关键词：Peptides ; Peptide conjugates ; Self-assembly ; Fluorescence
• 刊名：Colloids and Surfaces B: Biointerfaces
• 出版年：2016
• 出版时间：1 January 2016
• 年：2016
• 卷：137
• 期：Complete
• 页码：104-108
• 全文大小：1466 K

文摘

Derivatives of fluorophore FITC (fluorescein isothiocyanate) are widely used in bioassays to label proteins and cells. An N-terminal leucine dipeptide is attached to FITC, and we show that this simple conjugate molecule is cytocompatible and is uptaken by cells (human dermal and corneal fibroblasts) in contrast to FITC itself. Co-localisation shows that FITC-LL segregates in peri-nuclear and intracellular vesicle regions. Above a critical aggregation concentration, the conjugate is shown to self-assemble into beta-sheet nanostructures comprising molecular bilayers.