PNA-COMBO-FISH: From combinatorial probe design in silico to vitality compatible, specific labelling of gene targets in cell nuclei
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- 作者：Patrick Mü ; llera ; Jens Rö ; ß ; lera ; Jutta Schwarz-Finsterlea ; Eberhard Schmitta ; b ; eschmitt@kip.uni-heidelberg.de" class="auth_mail" title="E-mail the corresponding author ; Michael Hausmanna ; hausmann@kip.uni-heidelberg.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Fluorescence DNA labelling ; Peptide nucleic acids ; Hoogsteen triple strand binding ; Chemical oligonucleotide synthesis ; Confocal laser-scanning microscopy ; Combinatorial sequence search
- 刊名：Experimental Cell Research
- 出版年：2016
- 出版时间：1 July 2016
- 年：2016
- 卷：345
- 期：1
- 页码：51-59
- 全文大小：1126 K
文摘
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Denaturation free protocols preserve 3D architecture of chromosomes and nuclei.
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Labelling sets are determined in silico for duplex and triplex binding.
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Probes are produced chemically with freely chosen backbones and base variants.
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Peptide nucleic acid backbones reduce hindering charge interactions.
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Intercalating side chains stabilize binding of short oligonucleotides.