Development of fluorescence expression tools to study host-mycoplasma interactions and validation in two distant mycoplasma clades
详细信息 查看全文
- 作者：Tiffany Bonnefoisa ; b ; tiffany.bonnefois@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Marie-Sté ; phanie Vernereyc ; marie-stephanie.vernerey@supagro.inra.fr" class="auth_mail" title="E-mail the corresponding author ; Valé ; rie Rodriguesa ; b ; valerie.rodrigues@cirad.fr" class="auth_mail" title="E-mail the corresponding author ; Philippe Totté ; a ; b ; philippe.totte@cirad.fr" class="auth_mail" title="E-mail the corresponding author ; Carinne Puecha ; b ; carinne.puech@cirad.fr" class="auth_mail" title="E-mail the corresponding author ; Chantal Ripolld ; chantal.ripoll@inserm.fr" class="auth_mail" title="E-mail the corresponding author ; Franç ; ois Thiaucourta ; b ; francois.thiaucourt@cirad.fr" class="auth_mail" title="E-mail the corresponding author ; Lucí ; a Manso-Silvá ; na ; b ; lucia.manso-silvan@cirad.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：cfu ; colony forming units ; GFP ; green fluorescent protein ; MFI ; median fluorescence intensity ; mRFP1 ; monomeric red fluorescent protein ; MOI ; multiplicity of infection ; Mmm ; Mycoplasma mycoides subsp. mycoides ; PBS ; phosphate buffered saline ; PMT1 ; photomultiplier tube 1 ; PFA ; paraformaldehyde ; PI ; propidium iodide ; RT ; room temperature ; VSD ; variable secondary dichroic
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：20 October 2016
- 年：2016
- 卷：236
- 期：Complete
- 页码：35-44
- 全文大小：1297 K
文摘
- •
Development of tools for stable and innocuous whole mycoplasma cell labelling.
- •
Expression of bright red and green fluorescent proteins in two distant mycoplasmas.
- •
First observation of fluorescent mycoplasma colonies by fluorescence microscopy.
- •
Direct detection of fluorescent mycoplasmas in live host cells by flow cytometry.
- •
Visualisation of individual mycoplasmas inside host cells by confocal microscopy.