Quantification of hydrogen peroxide in plant tissues using Amplex Red
详细信息 查看全文
- 作者：Sourav Chakrabortya ; schakraborty@ccsu.edu" class="auth_mail" title="E-mail the corresponding author ; Amy L. Hilla ; 1 ; Gautam Shirsekara ; 2 ; Ahmed J. Afzalb ; 3 ; Guo-Liang Wanga ; David Mackeyb ; Pierluigi Bonelloa
- 关键词：ROS ; reactive oxygen species ; RFU ; relative fluorescence units (Ex/Em ; 560 ; nm/590 ; nm) ; AC ; activated charcoal ; AR ; Amplex Red ; PE ; plant extract ; PVPP ; polyvinylpolypyrrolidone ; TCA ; trichloroacetic acid ; NTCA ; neutralized trichloroacetic acid ; PB ; phosphate buffer ; DMSO ; dimethyl sulfoxide ; HRP ; horseradish peroxidase ; LoD ; limit of detection ; LoQ ; limit of quantitation ; CV ; coefficient of variation ; % RSD ; percent relative standard deviation (CV) ; SE ; standard error of the mean
- 刊名：Methods
- 出版年：2016
- 出版时间：15 October 2016
- 年：2016
- 卷：109
- 期：Complete
- 页码：105-113
- 全文大小：1028 K
文摘
- •
A reliable H2O2 quantitation method from plant cell lysate using Amplex Red is proposed.
- •
Commonly used extraction additives interfered with Amplex Red assay directly or indirectly.
- •
Constitutive and induced levels of H2O2 from three plants pine, rice and Arabidopsis is reported.
- •
Testing the effect of plant extract matrix on Amplex Red is critical for H2O2 quantitation.
- •
Amplex Red working solution is stable over a month under appropriate storage conditions.