A specific, rapid, cost-effective, and label-free fluorescence aptasensor based on target-responsive hydrogel was developed for rapid detection of avian influenza virus (AIV) H5N1.
The size-dependent property of this aptasensor was examined quantitatively and qualitatively.
QDs were embedded in this target-responsive DNA polymer crosslinked hydrogel, working as fluorescent reporters.
The total detection time from sampling to results was 30 min.