FRET biosensors reveal AKAP-mediated shaping of subcellular PKA activity and a novel mode of Ca2 +/PKA crosstalk
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- 作者：Micah B. Schott ; Faith Gonowolo ; Benjamin Maliske ; Bryon Grove ; bryon.grove@med.und.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：AKAP ; A-Kinase Anchoring Protein ; AKAR3 ; A-Kinase activity reporter 3 ; β2AR ; β2-adrenergic receptor ; [Ca2 ; +]i ; intracellular calcium concentration ; CaM ; calcium-calmodulin ; cAMP ; cyclic adenosine monophosphate ; CB4 ; calmodulin binding domain 4 ; CFP ; cyan fluorescent protein ; ECFP ; enhanced cyan fluorescent protein ; EGFP ; enhanced green fluorescent protein ; EYFP ; enhanced yellow fluorescent protein ; FRET ; Fö ; rster's resonance energy transfer ; Fsk ; forskolin ; GPCR ; G protein coupled recep
- 刊名：Cellular Signalling
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：28
- 期：4
- 页码：294-306
- 全文大小：2775 K
文摘
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The FRET biosensor AKAR3 was used to investigate gravin's impact on PKA activity.
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Gravin elevated plasma membrane PKA activity and reduced cytosolic PKA activity.
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Gravin is redistributed away from the cell periphery upon Ca2 + elevation.
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Ca2 + elevation reduced plasma membrane PKA activity through gravin redistribution.