Imaging the microenvironment of pancreatic cancer patient-derived orthotopic xenografts (PDOX) growing in transgenic nude mice expressing GFP, RFP, or CFP
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- 作者：Robert M. Hoffmana ; b ; all@anticancer.com" class="auth_mail" title="E-mail the corresponding author ; Michael Bouvetb
- 关键词：Patient-derived orthotopic xenografts (PDOX) ; Nude mice ; Green fluorescent protein ; Red fluorescent protein ; Cyan fluorescent protein ; Tumor microenvironment
- 刊名：Cancer Letters
- 出版年：2016
- 出版时间：28 September 2016
- 年：2016
- 卷：380
- 期：1
- 页码：349-355
- 全文大小：3422 K
文摘
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Transgenic nude mice expressing different colored fluorescent proteins are used as hosts for patient tumors.
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Fluorescent stroma is stably associated with the patient tumors growth in colored nude mice and subsequent passage.
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Multiple colors of nude mice allow color coding of stromal cells associated with the patient tumors.
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Colored stroma allows real time imaging of the tumors noninvasively and intravitally at the cellular level.
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The cancer cells within the PDOX tumors can be labeled with a GFP-containing telomerase-dependent adenovirus.