Photophysical studies on a photoactive yellow protein fluorophore analog with the 4-Hydroxy group replaced by 4-Dimethylamino group

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• 作者：Giasuddin Ahamed^a ; Shaikh Batuta^b ; Deborin Ghosh^a ; Naznin Ara Begum^b ; Debabrata Mandal^a ; ^{dmandal.chemistry@gmail.com}
• 刊名：Journal of Photochemistry and Photobiology A: Chemistry
• 出版年：2017
• 出版时间：15 February 2017
• 年：2017
• 卷：335
• 期：Complete
• 页码：86-93
• 全文大小：1132 K
• 卷排序：335

文摘

A highly polarity sensitive PYP Fluorophore analog – pDMACT – has been prepared, exhibiting fluorescence Stokes shifts comparable to PRODAN. Unlike previous analogs, here the p-phenolate group is replaced – instead of the carbonyl end group – by p-N,N-dimethylaminophenyl group. pDMACT is thus a prototype for new fluorescent labels that can be attached to a biomolecule by the thioester linkage. Its structure promotes strong intramolecular charge transfer, which plays a major role in its excited state relaxation in bulk solvents.