- 作者：Qing Tang ; Fanghong Shang ; Xiaocui Wang ; Yang Yang ; Gang Chen ; Yi Chen ; Jifen Zhang ; Xiaoyu Xu
- 关键词：FA ; Ferulic acid ; LZ ; Ligustrazine ; THP ; Tetrahydropalmatine ; GnRH ; Gonadotropin-releasing hormone ; FSH ; Follicle-stimulating hormone ; LH ; Luteinizing hormone ; E2 ; Estradiol ; ER ; Estrogen receptor ; HSP90 ; Heat-shock protein 90 ; COX-2 ; Cycloxygenase-2 ; ERE ; Estrogen response element ; TNF-伪 ; Tumor necrosis factor ; IL-1尾 ; Interleukin-1尾 ; ELISA ; Enzyme linked immunosorbent assay ; NF-魏B ; Nuclear transcription factor-魏B ; I魏B伪 ; Inhibitor of nuclear factor-魏B ; 尾-actin ; An internal control used
- 刊名：Journal of Ethnopharmacology
- 出版年：12 February, 2014
- 年：2014
- 卷：151
- 期：3
- 页码：1218-1225
- 全文大小：5795 K
Ethnopharmacological relevance
Ferulic acid (FA), ligustrazine (LZ) and tetrahydropalmatine (THP) are separately isolated from Chinese Angelica, Szechwan Lovage Rhizome and Rhizoma in the Jiawei-Foshou-San formula, a popular traditional Chinese medicine for irregular menses. It has been reported that the combination use of FA+LZ+THP has similar effect on endometriosis, but the underlying mechanisms are unclear. This study was to investigate the combination effects and mechanisms of FA+LZ+THP on endometriosis rats.Materials and methods
Fifty endometriosis rats were intragastricly treated with FA+LZ+THP for 4 wk. The volume of ectopic endometrial tissue was measured to evaluate the effects. Then the changes in hypothalamic-pituitary-ovarian axis and ERE pathway were indicated by the levels of E2, GnRH, FSH and LH, and the expressions of ER, HSP90 and COX-2, respectively. In addition, peritoneal macrophages of each rat were cultured in vitro and treated with (FA+LZ+THP)-medicated serum for 24 h. The proliferation and phagocytosis abilities, the levels of IL-1尾 and TNF-伪, and the expression of I魏B伪 were then measured for the changes of peritoneal macrophage activities.
Results
Combination use of FA+LZ+THP diminished the volume of the ectopic endometrial tissues (P<0.05 or P<0.01). It also decreased the E2 level, suppressed the expression of GnRH, FSH and LH, and decreased the protein expression of ER, HSP90 and COX-2 (all P<0.05 or P<0.01). The phagocytosis ability of peritoneal macrophage was enhanced by (FA+LZ+THP)-medicated serum (P<0.05) with no change of proliferation (P>0.05). Moreover, IL-1尾 and TNF-伪 were downregulated (both P<0.05 or P<0.01) and I魏B伪 was upregulated by the (FA+LZ+THP)-medicated serum (P<0.01).
Conclusions
The combination use of FA, LZ and THP could inhibit the growth of ectopic endometrial tissue in endometriosis rats. It might be related to the down-regulation of hypothalamic-pituitary-ovarian axis, the amelioration in ERE pathway and the improvement of peritoneal macrophage activities.